Category 'Scientific Resources'

  1. New resources from antibodies-online

    At CiteAb we work hard to get to know individual antibody suppliers so that you can be sure the data we hold is always up-to-date and correct. Every now and then we feature individual company highlights here on our blog, allowing you to get to know leading antibody suppliers too. This week we’re featuring antibodies-online, […]

  2. Some great research resources from Bio-Rad AbD Serotec

    At CiteAb we work hard to get to know individual antibody suppliers so that you can be sure the data we hold is always up-to-date and correct. Every now and then we feature individual company highlights here on our blog, allowing you to get to know leading antibody suppliers too. We recently spoke to Bio-Rad […]

  3. Detergents as cell permeabilisation reagents: Saponin vs Tween-20 vs Triton-x

    Detergents are used in a broad range of scientific procedures but they often seem to be used interchangeably and figuring out why one is used over another in different applications can be quite confusing. In this post we’re going to concentrate on the use of detergents for permeabilisation of cell membranes in intracellular staining. If […]

  4. Detergents as cell permeabilisation reagents

    Detergents are used in a wide range of experiments and sometimes figuring out which one to use can be quite tricky (something we take a closer look at here). This choice can be made even more confusing if you’re unsure as to how detergents actually work! Why permeabilise cell membranes? In order to visualise the […]

  5. Comparing fixing agents: methanol vs acetone for IF

    Immunofluorescence is a powerful technique that uses fluorescently labelled antibodies to detect target antigens, but the choice of fixation method can make a big difference to the results obtained. When we fix cells, the goal is to maintain the structure as close to the native state as possible, but to also still allow antibodies to […]

  6. What are the most used loading controls for Western Blotting?

    Loading control antibodies are some of the most utilised antibodies in research. Antibodies against several different targets are commonly used and it’s not always obvious which you should choose. We provide a guide to some of the most commonly used types of loading control antibodies. Why do I need a loading control for Western blotting? […]

  7. CiteAb data shows rise in use of Chromatin Immunoprecipitations

    Our latest data from CiteAb suggests that the use of Chromatin Immunoprecipitation (ChIP) has risen significantly over the last 15 years. Here at CiteAb we amass data from the analysis of hundreds of thousands of life sciences publications that use research antibodies, giving a powerful and unique insight into antibody use and market trends. Today’s […]

  8. New tool changes research collaboration

    In an exciting step to simplify the process of collaborating and writing new research, F1000 has today announced the launch of innovative new tool F1000Workspace. As you’ll be aware, here at CiteAb we aim to enhance the effectiveness of research and to ensure that researchers have the tools required to make good decisions about the […]

  9. Western blotting – Milk vs BSA for blocking

    Western blotting is one of the most common experiments performed in the laboratory using antibodies. A common question when performing a western blot is “Which blocking agent should I use – Milk or BSA?” This week we are going to look at the pros and cons of Milk and BSA and when you should use […]

  10. The Antibody Validation Collection – for more reliable antibodies

    We’re pleased to announce that Andrew and Matt here at CiteAb have been working with F1000Research, resulting in the recent launch of the Antibody Validation Collection, enhancing the reliability and reproducibility of antibodies in scientific research. Antibodies are one of the most used tools in any life sciences laboratory; a survey conducted by The Scientist […]